Humming, Chanting and Om: What the Evidence Shows
Yakiv Bilenko — editor · Updated October 8, 2026

Humming, chanting and singing are vocal practices done on a long exhale. Studied effects include a large rise in nasal nitric oxide during humming, slower breathing with higher vagally mediated heart rate variability during practice, and small benefits for stress and wellbeing in some trials. Most studies are small and unblinded. The evidence does not by itself establish that the sound or vibration adds anything beyond slower breathing.
Key points
- Humming sharply raises nitric oxide measured in nasal air, because the sound vibration speeds up gas exchange with the sinuses.
- A rise in nasal nitric oxide during humming has not been shown to prevent or treat sinus disease.
- Chanting, mantras and singing slow the breath, and slow breathing is associated with higher vagally mediated heart rate variability during practice.
- Whether the hum or vibration adds an effect beyond slowing the breath has not been shown.
- The often-cited Om brain-imaging study was a small pilot without independent replication.
- Mantra meditation shows small-to-moderate benefits for anxiety and stress in randomized trials, with a risk of bias.
- Claims that Om matches a cosmic frequency have no scientific basis in the studies reviewed here.
What are humming, chanting and Om?
They are vocal practices done on the out-breath. Researchers have studied four of them separately, and their results should not be merged:
- Humming on the exhale — in yoga, bhramari or "bee breath": a steady "mmm" through the nose on a long exhale.
- Om chanting — the syllable sung aloud on one breath, ending in a hum.
- Mantra recitation — repeating a phrase, aloud or silently; some trials also include prayers such as the rosary.
- Choir singing — group singing of songs with set phrases.
What they share is a slowed breath with a long, voiced exhale. What differs is the sound, the setting, the meaning to the person and, above all, what was measured.
How does it work?
Two physical things happen when you hum or chant.
The breath slows. A sung phrase or a long hum stretches the exhale, so the whole breathing cycle slows. Slow breathing is associated with higher vagally mediated HRV; whether a longer exhale adds anything beyond slowing the breath is still debated [S6]. The way breathing shapes heart rhythm is covered in breathing and HRV.
The air vibrates. A hum makes the air in the nose oscillate. In physical models this oscillation pushes air in and out of the narrow openings of the paranasal sinuses (the air-filled cavities around the nose), flushing out gas stored inside [S4]. The sinuses are a major source of the nitric oxide found in nasal air [S1] [S5].
The vagus nerve is often named as a third route. Its branches do supply the voice box: in the neck it innervates most muscles of the larynx and pharynx, which serve voice and swallowing [S11]. That is anatomy, not a demonstrated mechanism. No study we found showed that the vibration of a hum changes vagal signals to the heart on top of the slower breathing. Background is in the vagus nerve.
How is it measured?
Different outcomes need different tools, and each study used only some of them:
- Nasal nitric oxide — measured in exhaled nasal air with a chemiluminescence analyser, during humming and during quiet exhalation at the same flow [S1].
- Heart rate and HRV — from ECG or a pulse sensor, during and after the practice [S7] [S9].
- Breathing rate — counted or recorded with a belt [S7] [S8].
- Brain activity — functional MRI, which tracks blood-flow changes, not nerve firing [S12].
- Mood, anxiety and wellbeing — questionnaires, which are open to expectation effects because participants know what they are doing [S13] [S14].
What affects it?
- Breathing rate. In the mantra study the effect was tied to reciting at a set pace [S7]. A chant that does not slow the breath may not do the same.
- Sinus anatomy. For nitric oxide, the size of the sinus opening mattered most, and the hum's pitch also played a part [S2].
- Repetition. Repeated hums empty the sinuses of stored nitric oxide; levels recover after a quiet pause [S2] [S3].
- Health status. In people with nasal polyps, the post-humming change seen in healthy people did not appear [S3].
- Setting and meaning. Group singing adds social contact, and prayer or mantra adds belief and focus. Studies rarely separate these from the breathing.
What does the evidence show?
Nasal nitric oxide — physiology shown, health benefit unknown
In a study of ten healthy volunteers, nasal nitric oxide rose about fifteen-fold during a single breath of humming compared with quiet exhalation at the same fixed flow rate; a physical model of the nose and sinus was used to explain the rise [S1]. This was a measurement in healthy people, not a treatment study. This is a single small study, but the same research group confirmed the large rise and traced it to rapid gas exchange in the sinuses (class: emerging for the size, context-dependent for the mechanism) [S2] [S4].
What follows: humming ventilates the sinuses for as long as you hum, and the effect has been used as a research test of sinus ventilation [S1] [S2]. What does not follow: the authors wrote that therapeutic effects "should be investigated" [S1]. We found no controlled trial showing that humming prevents or treats sinusitis or infections (class: unknown). How nitric oxide behaves in nasal versus mouth breathing is covered in nasal breathing.
Breathing and HRV — explained by slow breathing so far
Voluntary slow breathing raises vagally mediated HRV during practice, across a large meta-analysis of 223 studies (class: established) [S6]. Chanting and singing are forms of slow, guided breathing, so a rise in HRV during them is expected. More detail is in slow breathing: the evidence.
In a 2001 laboratory study, twenty-three healthy adults recited the rosary or a yoga mantra. Reciting six times a minute set breathing to a similarly slow pace, and cardiovascular rhythms became stronger and synchronised (class: emerging, single study) [S7]. The authors credited the breathing rhythm, not the words.
In choir singers, song structure guided breathing, and unison singing made heart rates rise and fall together (class: emerging, single small study) [S8].
Is there an effect beyond slowing the breath? In a randomized trial of seventy people with essential hypertension, one five-minute session, bee-humming breathing was compared with slow breathing. Blood pressure did not fall more after humming; some spectral HRV indices differed during recovery, but their autonomic meaning is debated (class: emerging, single trial) [S9]. A systematic review of bhramari found six studies, none of them randomized, and rated their quality as very low [S10]. So an added effect of the hum itself has not been shown (class: unknown).
The brain — one small pilot
In a pilot fMRI study of twelve healthy volunteers, audible Om chanting was accompanied by lower activity in limbic regions, including the amygdala and hippocampus, compared with rest; a "ssss" sound showed no such change [S12]. It was a pilot, without blinding, and we found no independent replication. The authors compared the pattern with vagus nerve stimulation as a hypothesis, not a measured route (class: emerging).
Outcomes, one at a time
- Stress and anxiety. In randomized trials, mantra-based meditation produced small-to-moderate reductions in anxiety, depression and stress compared with controls, weakened by risk of bias (class: context-dependent) [S13]. Most of these trials used silent mantra techniques, not audible chanting.
- Blood pressure. One session of humming did not lower blood pressure more than slow breathing [S9]. Long-term trials of humming alone are lacking (class: unknown). Slow breathing in general is covered in slow breathing.
- Sleep. We found no controlled trial of humming or chanting with sleep as the main outcome (class: unknown).
- Mood and wellbeing from singing. A systematic review found reliable evidence for positive effects of music and singing on wellbeing in adults (class: context-dependent) [S14]. Measures varied widely, and choir members tended to be female, white and relatively well educated.
- Meditation more broadly. Effects of meditation on autonomic measures are in meditation and the autonomic nervous system.
What it does not tell you
- Sound frequency claims are not supported. A popular idea is that Om matches a cosmic or "healing" frequency, often quoted as a specific number of hertz. None of the studies reviewed here tested or supported this; it has not been shown, and no study we found measured health effects of a particular pitch.
- "Humming works the vagus harder than other practices" has not been shown. No study we found compared vagal effects of humming with silent slow breathing at the same pace. The one controlled comparison found no blood-pressure advantage [S9]. The framing matters: Vagal tone cannot be measured directly; HRV measures such as RMSSD reflect vagally mediated changes in heart rate.
- Small samples, no blinding. Most studies had a few dozen people or fewer. Participants know when they hum or pray, so expectation can shape questionnaire results.
- One study is not a rule. The nitric oxide, mantra and Om imaging findings each rest mainly on a single small study.
- Nasal nitric oxide is not a health score. A higher value during humming says the sinuses are ventilating, not that you are healthier.
- Symptoms belong with a doctor. Long-lasting facial pain, nasal blockage or fever, as well as chest pain, fainting or severe breathlessness, need medical assessment, not a breathing practice.
Conflicts of interest. The Om imaging study came from a yoga centre within a psychiatric institute [S12], and the bee-humming trial was partly run by an integrative-medicine centre [S9]; both declared no conflicts. The nitric oxide studies came from one research network, and the mantra meta-analysis and singing review declared no conflicts [S13] [S14].
In ONDA
ONDA cares about humming and chanting because they are slow, guided breathing, which shapes heart rhythm. ONDA shows pulse during practice from the phone camera or Apple Watch, an estimated breathing rate, and a coherence score with an Apple Watch only. The app has no humming-specific practice or measurement, does not measure nitric oxide or brain activity, and does not diagnose anything. Practical guides are in humming breath and Om chanting.
Educational information, not a diagnosis or medical treatment.
Evidence at a glance
| Claim | Evidence | Limitation |
|---|---|---|
| Nasal nitric oxide rose sharply during single-breath humming compared with quiet exhalation at a fixed flow, in healthy volunteers. [S1] | Emerging | Single small study in healthy adults; confirmed in direction by the same group (S2), not an outcome study. |
| The rise comes from faster gas exchange between the sinuses and the nasal cavity; ostium size matters most. [S2][S4] | Context-dependent | Small human study and physical models from one research network. |
| Repeated humming empties the sinuses of stored nitric oxide; levels recover after a quiet pause. [S2][S3] | Emerging | Measurement study; says nothing about health effects. |
| The authors proposed humming nitric oxide as a test of sinus ventilation and said therapeutic effects should be investigated, not that they were shown. [S1] | Unknown | No controlled trial of humming for sinus disease was found. |
| The paranasal sinuses are a major source of the nitric oxide found in nasal air. [S5][S1] | Established | Physiology; does not by itself establish a benefit of raising it. |
| Voluntary slow breathing increases vagally mediated HRV during practice, across a large meta-analysis. [S6] | Established | About slow breathing in general, not humming or chanting specifically. |
| Reciting a rosary prayer or a mantra slowed breathing to about six breaths a minute and strengthened existing cardiovascular rhythms in healthy adults. [S7] | Emerging | Twenty-three healthy adults, no blinding; the authors attribute the effect to the breathing rhythm. |
| In singers, song structure guides breathing, and unison singing makes heart rates rise and fall together. [S8] | Emerging | Small study in young adults; the authors frame singing as guided breathing. |
| A single five-minute session of bee-humming breathing did not lower blood pressure more than slow breathing in people with hypertension; some HRV indices differed afterwards. [S9] | Emerging | One trial, one session, spectral HRV indices whose autonomic meaning is debated. |
| Studies of bhramari were few, heterogeneous, of very low methodological quality and not randomized as of the review. [S10] | Unknown | Review searched studies only up to May 2016. |
| In the neck, the vagus nerve supplies most muscles of the larynx and pharynx, used for voice and swallowing. [S11] | Established | Anatomy; motor supply to the voice muscles does not show that vocal vibration changes vagal output to the heart. |
| In a pilot fMRI study, audible Om chanting was accompanied by deactivation of limbic regions compared with rest, and a 'ssss' control showed no such change. [S12] | Emerging | Twelve volunteers, pilot design, no independent replication found; the vagal route is the authors' hypothesis. |
| Mantra-based meditation produced small-to-moderate reductions in anxiety, depression and stress compared with controls, weakened by risk of bias. [S13] | Context-dependent | Mostly transcendental meditation; silent mantra repetition, not audible chanting. |
| A systematic review found reliable evidence for positive effects of music and singing on wellbeing in adults. [S14] | Context-dependent | Mixed music and singing; inconsistent wellbeing measures; choir members tend to be female, white and well educated. |
Sources
- [S1] Weitzberg & Lundberg (2002). Humming greatly increases nasal nitric oxide. American Journal of Respiratory and Critical Care Medicine. DOI 10.1164/rccm.200202-138BC · PMID 12119224 · Small experimental study in ten healthy volunteers plus a physical model
- [S2] Maniscalco et al. (2003). Assessment of nasal and sinus nitric oxide output using single-breath humming exhalations. European Respiratory Journal. DOI 10.1183/09031936.03.00017903 · PMID 12952268 · Small experimental study in healthy volunteers plus a sinus model
- [S3] Maniscalco et al. (2003b). Nasal nitric oxide measurements before and after repeated humming maneuvers. European Journal of Clinical Investigation. DOI 10.1111/j.1365-2362.2003.01277.x · PMID 14636292 · Includes people with allergic rhinitis and nasal polyposis; small groups
- [S4] Granqvist et al. (2006). Paranasal sinus ventilation by humming. Journal of the Acoustical Society of America. DOI 10.1121/1.2188887 · PMID 16708919 · Physical tube model and computer model, no human participants
- [S5] Lundberg (2008). Nitric oxide and the paranasal sinuses. The Anatomical Record. DOI 10.1002/ar.20782 · PMID 18951492
- [S6] Laborde et al. (2022). Effects of voluntary slow breathing on heart rate and heart rate variability: a systematic review and a meta-analysis. Neuroscience & Biobehavioral Reviews. DOI 10.1016/j.neubiorev.2022.104711 · PMID 35623448
- [S7] Bernardi et al. (2001). Effect of rosary prayer and yoga mantras on autonomic cardiovascular rhythms: comparative study. BMJ. DOI 10.1136/bmj.323.7327.1446 · PMID 11751348 · Small laboratory study in healthy adults
- [S8] Vickhoff et al. (2013). Music structure determines heart rate variability of singers. Frontiers in Psychology. DOI 10.3389/fpsyg.2013.00334 · PMID 23847555 · Small study in eighteen-year-olds plus a five-person case study; erratum published
- [S9] Ghati et al. (2021). A randomized trial of the immediate effect of Bee-Humming Breathing exercise on blood pressure and heart rate variability in patients with essential hypertension. EXPLORE. DOI 10.1016/j.explore.2020.03.009 · PMID 32620379 · Single session; partly run by an integrative-medicine (yoga) research centre
- [S10] Kuppusamy et al. (2018). Effects of Bhramari Pranayama on health - A systematic review. Journal of Traditional and Complementary Medicine. DOI 10.1016/j.jtcme.2017.02.003 · PMID 29321984
- [S11] Breit et al. (2018). Vagus nerve as modulator of the brain-gut axis in psychiatric and inflammatory disorders. Frontiers in Psychiatry. DOI 10.3389/fpsyt.2018.00044 · PMID 29593576
- [S12] Kalyani et al. (2011). Neurohemodynamic correlates of 'OM' chanting: A pilot functional magnetic resonance imaging study. International Journal of Yoga. DOI 10.4103/0973-6131.78171 · PMID 21654968 · Pilot study from a yoga centre within a psychiatric institute; twelve volunteers
- [S13] Álvarez-Pérez et al. (2022). Effectiveness of Mantra-Based Meditation on Mental Health: A Systematic Review and Meta-Analysis. International Journal of Environmental Research and Public Health. DOI 10.3390/ijerph19063380 · PMID 35329068
- [S14] Daykin et al. (2018). What works for wellbeing? A systematic review of wellbeing outcomes for music and singing in adults. Perspectives in Public Health. DOI 10.1177/1757913917740391 · PMID 29130840
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